Glycerol-Free RNase H (HC) is an RNA-specific endonuclease that hydrolyses the phosphodiester bonds of RNA, when hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. It does not hydrolyse the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Glycerol-Free RNase H (HC) is a high concentration (50 U/µL) RNA-specific endonuclease that hydrolyses the phosphodiester bonds of RNA, when hybridised to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA. It does not hydrolyse the phosphodiester bonds within single-stranded and double-stranded DNA and RNA. Glycerol-Free RNase H (HC) has been optimised to deliver excellent, stable performance without glycerol, allowing you to deliver reliable results with the added capability for lyophilisation. This gives you the flexibility to confidently store reaction mixtures at ambient temperature or produce diagnostic assays that rely on miniaturised reaction components.
- Removal of RNA template following second-strand cDNA synthesis
- Removal of mRNA poly(A) sequence after hybridisation with oligo(dT)
- Ideal for nucleic acid sequence-based amplification (NASBA)
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