Lyo-Ready Direct DNA LAMP and RNA/DNA LAMP Urine mixes are ready-to-use, 4x concentrated, fully optimised mixes for isothermal amplification methods such as loop mediated isothermal amplification (LAMP) on urine samples, designed to dramatically speed up assay development time and reduce time to results. The glycerol-free mixes contain optimised excipients compatible with freezing and lyophilisation, making them ideal for POC urine testing. Can also be used wet and/or with purified nucleic acid.

Urinary tract infections (UTIs) affect over 150 million people worldwide and are the second most common cause of infection in primary and secondary care and most women experience at least one episode of acute uncomplicated cystitis in their lifetime. UTIs are responsible for a major part of antibiotic prescriptions, accounting for up to 15% of antimicrobial use in the community, with antibiotic use been described as one of the main factors contributing to the emergence of antimicrobial resistance. With the stigma attached to sexually transmitted diseases (STDs), and with their rapid spread in the last few years, there is also a need for discrete, fast and accurate point-of-care (POC) tests as well.

The Lyo-Ready Direct DNA LAMP Urine and Lyo-Ready RNA/DNA LAMP Urine mixes combine even greater tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crude urine samples. The mixes are uniquely designed to overcome much higher concentrations of inhibitors, and so do not require a urine DNA or urine RNA purification step to analyse a patient sample. Very fast reaction kinetics result in faster time to results and superior amplification sensitivity and specificity. In addition, the 4x concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient-temperature stable assays, making them ideal for POC urine testing.

• Ideal for developing screening assays for sexually transmitted diseases (STDs) and urinary tract infections (UTIs)
• Very sensitive detection of low copy number bacteria, viruses and cell-free nucleic acids from crude urine samples
• Quickly and accurately detect slow-growing and difficult-to-culture microorganisms, removing ambiguous results
• Can be used wet or lyophilised, ideal for developing ambient-temperature stable POC assays