Lyo-Ready Direct DNA LAMP and RNA/DNA LAMP Blood mixes are ready-to-use, 4x concentrated, fully optimised mixes for isothermal amplification methods such as loop-mediated isothermal amplification (LAMP) on blood. They are designed to dramatically speed up assay development time and reduce time to results. The glycerol-free mixes contain optimised excipients compatible with freezing and lyophilisation, making them ideal for POC blood testing. Can also be used wet and/or with purified nucleic acid.

Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders such as diabetes. However, whole blood specimens, serum, and plasma contain a number of inherent PCR inhibitors including immunoglobulin G, haemoglobin, lactoferrin, and leukocyte DNA. In addition, PCR inhibitors can be found in the anticoagulants used to stabilise blood samples (e.g. EDTA, citrate, or heparin). Isothermal amplification such as LAMP is very tolerant to inhibitors and so is ideal for point of care (POC) testing, the simple workflow allows fast, sensitive and reliable results in clinical diagnosis.

The Lyo-Ready Direct DNA LAMP Blood and Lyo-Ready RNA/DNA LAMP Blood mixes combine even greater tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed whole blood, plasma, or serum samples. The mixes are uniquely designed to overcome much higher concentrations of inhibitors, and so do not require a DNA or RNA purification step to concentrate and purify a patient sample. Very fast reaction kinetics result in faster time to results and superior amplification sensitivity and specificity. In addition, the 4x concentrated master mixes are formulated with lyo-excipients which are optimised for freeze-drying to create ambient-temperature stable assays, making them ideal for POC blood testing.

• Develop ultra-sensitive LAMP blood tests from whole blood, plasma, and serum samples
• Shorten the time to results by removing the nucleic acid extraction
• Simplify the development of POC blood testing by just adding primers and probes
• Can be used wet or it lyophilised, ideal for developing blood pathogen and liquid biopsy assay