Ribonucleases (RNases) are ubiquitous and can be introduced into experiments in many ways: for example co-purification during RNA isolation, carryover from bare hands and pipette tips. This RNase contamination can often go unnoticed. RNase Inhibitor is ideal for RNA-sensitive applications such as RT-qPCR as even a small amount of RNase can be detrimental to the final experimental outcome.
Meridian RNase Inhibitors are from a recombinant protein that inhibits different RNases (A, B, C) by binding non-covalently in a 1:1 ratio. With an association constant of 1014 M, they are useful in any applications where the presence of RNases is a potential problem. RNase Inhibitor is tested for activity, SDS-PAGE purity, and the absence of endonucleases, nickases, and exonuclease. The glycerol-free formulation ensures the compatibility of RNase Inhibitor (Glycerol Free) to lyophilised formats.
- Inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C
- Free of contaminating DNases, RNases, phosphatases and nickases for improved sample security
- Active up to 55°C across a wide range of DTT concentrations and pH
- Compatible with lyophilisation in an assay mix for ambient temperature storage and shipment
This product has met the following criteria to qualify for the following awards: