A variety of PCR inhibitors inherent to clinical and environmental samples can negatively impact the sensitivity and accuracy of a molecular assay. Conventionally, to overcome this, specimens undergo processing before testing. However, extraction technologies are not 100% efficient and can impact the amount of target nucleic acid available for testing. Some PCR inhibitors can also co-elute following purification, reducing the efficiency, particularly in quantitative PCR multiplex assays and may even induce false-negative assay results.

Meridian’s universal Inhibitor-Tolerant qPCR Buffer is designed for developing qPCR multiplex assays that require minimal sample processing and fast turn-around times (TAT). Because the buffer is highly resistant to various qPCR inhibitors, it can be used with crude lysates or inhibitor-rich samples, to directly detect pathogens, including viruses and bacteria, in various biological specimens and to analyse gene expression in cells.

• Ready-to-use qPCR buffer engineered for amplification from crude samples without the need for complex or time-consuming extraction
• 5x concentration buffer allows for greater sample volume, simply add DNA polymerase, dNTPs, MgCl₂, primers, probes and clinical samples
• Ideal for developing quantitative PCR assays where sample is limited