Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) is an RNA-dependent DNA polymerase that can be used for cDNA synthesis and subsequent PCR or qPCR in one-step or two-step RT-PCR or RT-qPCR assays.
MMLV-RT is a high-quality reverse transcriptase that synthesises a complementary DNA (cDNA) strand from mRNA or total RNA. With higher thermostability and reduced RNase H activity, it can be used for synthesis with long messenger RNA templates with high sensitivity and efficiency and is ideal for detecting low-level target genes.
- Highly sensitive, even with low concentrations of template RNA
- Thermostability up to 45°C, for more challenging transcripts
- High-quality, first-strand cDNA ideal for most RT-PCR and RT-qPCR applications
- Lower RNase H activity for high yield
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