Meridian Bioscience RNase-Tolerant MMLV-RT - 50000 Reactions

RNase-Tolerant MMLV-RT is a reverse transcriptase/RNase inhibitor mix optimised for one-step RT-qPCR. RNase-Tolerant MMLV-RT is used for the detection of RNA at very low levels, it can also be used with a broader temperature range, making it ideal for applications such as blood bank or transplant viral testing.

A Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RT), which exhibits high sensitivity and efficiency, and RNase Inhibitor (Ribonuclease Inhibitor) which inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C. RNase-Tolerant MMLV-RT shows no inhibition of polymerase or reverse transcriptase activity, so can be used in cDNA synthesis or one-step RT-qPCR reactions. RNase-Tolerant MMLV-RT is useful in any applications where the presence of RNases is a potential problem.

• Highly sensitive cDNA synthesis, even low concentrations of template RNA
• Broader temperature range, up to 50°C, for more challenging transcripts
• High efficiency where samples are likely to contain inhibitors
• High-quality, first-strand cDNA synthesis ideal for most one-step RT-qPCR