Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesise DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and for Loop-Mediated Isothermal DNA Amplification (LAMP).

Isothermal amplification such as loop-mediated isothermal amplification (LAMP) is a DNA amplification technique that can be performed at a single temperature. LAMP is currently considered one of the most powerful isothermal amplification techniques, relying on a strand-displacement polymerase combined with four to six primers. These primers recognise several specific regions in the target DNA and two of the primers form loop structures to facilitate subsequent rounds of amplification producing high levels of DNA.

Meridian’s Bst polymerases have strong strand displacement activity, fast polymerisation, and enhanced inhibitor and salt tolerance when used with the specialised Inhibitor-Tolerant reaction buffer. The 100x high-concentration, glycerol-free BST enzyme was specifically designed to maximise assay flexibility for LAMP diagnostic test development.