High Conc. Glycerol-Free Bst is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesise DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA and, Loop-Mediated Isothermal DNA Amplification (LAMP).
High Conc. Glycerol-Free Bst (100 U/µL) is supplied with an Enzyme Dilution Buffer and a Bst Reaction Buffer formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance, and sensitivity, speeding up your product development time and allowing you to commercialise your new products faster. High Conc. Glycerol-Free Bst is also lyophilisation-compatible, making it ideal for the development of point of care test (POCT) assays that require ambient temperature shipping and storage.
- Optimised for isothermal amplification such as LAMP test
- Designed for fast polymerisation and so faster time to results
- Supplied with a reaction buffer and enzyme dilution buffer
- Ideal for creating lyophilised POCT assays
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