Taq HS Antibody binds Taq DNA polymerase and inhibits its activity until reaction temperature is elevated at the start of a PCR (hot start PCR). At that point, the Taq Antibody is denatured and releases its hold on Taq DNA polymerase, allowing DNA synthesis to proceed. This enhances the specificity and sensitivity of PCR by preventing primer-dimer formation and non-specific amplification at lower temperatures.
Taq HS Antibody is a mix of anti-Taq antibodies designed to inhibit Taq DNA polymerase activity at room temperature. It is used in the preparation of hot-start PCR or qPCR master mixes and helps to prevent nonspecific amplification and primer-dimer formation, increasing specificity and sensitivity while allowing assembly of reactions at ambient temperatures. Inhibition is reversed when the temperature is raised during the first denaturing step of thermal cycling.
- Anti-Taq antibody for hot start PCR
- Helps to prevent mispriming and non-specific amplification derived from primer dimers
- Can be used under conventional PCR conditions
- Glycerol-free, ideal for preparation of dried-down PCR tests
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