A highly inhibitor-resistant RT-qPCR 4x master mix that provides quick and easy extraction and amplification of DNA from a variety of tissue types. The supermix maximises sensitivity while simultaneously minimising the effect of blood, tissue and plant PCR inhibitors, to deliver greater experiment success rates. SensiFAST™ Probe One-Step Direct Lo-ROX SuperMix is a 4x, combination of the latest advances in buffer chemistry and PCR enhancers and stabilisers, together with an antibody-mediated hot-start polymerase, reverse transcriptase, RNase Inhibitor, dNTPs and MgCl₂. The advanced buffer chemistry and enhancers has been developed for fast RT-qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan®, Scorpions® and molecular beacon probes, and has been validated on all commonly used real-time instruments that require a low concentration of the passive reference dye ROX, making SensiFAST Direct Probe One-Step Direct Lo-ROX SuperMix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.

• Robust: optimised proprietary buffer system designed for overcoming common PCR inhibitors in crude lysate or unprocessed blood, tissue and plant samples
• Specific: antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
• Sensitive: reliable quantification of low abundance targets and scarce samples
• Reproducible: consistent results between technical replicates for increased confidence in results
• Fast: delivers reproducible, multiplex accurate assay results in as little as 30 minutes