The SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes . A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR . SensiFAST Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.

Features and Benefits

• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Sensitive – perfect for hard to obtain and low copy number samples
• High performance - ideal for multiplexing and gene expression analysis
• Fast protocols - use with the new generation of fast cyclers

Instrument Compatibility: ABI 7500, 7500 FAST, ViiA7™, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, as well as other instruments that do not require the use of a passive reference.

Volume: BIO-84002: 250 x 20µl Reactions: 2 x 1ml BIO-84005: 500 x 20µl Reactions: 5 x 1ml BIO-84020: 2000 x 20µl Reactions: 20 x 1ml

Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2, ROX Internal reference

Kit Size: The kit size is based on a 20µl final reaction volume.

Storage Conditions: 12 months at -20°C. Avoid exposure of the ROX™ to light.