The SensiFAST SYBR Lo-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on many commonly used real-time instruments. The kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR Lo-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR . The SensiFAST SYBR Low-ROX One-Step Kit consists of a 2x SensiFAST SYBR One-Step mix, which contains ROX for optional use, separate reverse transcriptase and RiboSafe RNase Inhibitor.

Features and Benefits

• Rapid - hot start capability saves time and reduces primer-dimer formation
• Reproducible – Optimized reverse-transcription and amplification buffer
• Accurate quantification - of gene expression over a broad dynamic range
• Sensitive – unique buffer chemistry for highest specificity

Instrument Compatibility: ABI 7500, 7500 FAST, Stratagene (Agilent) Mx4000™, Mx3000P™, Mx3005P™, (See product selection table), as well as other instruments that do not require the use of a passive reference.

Volume BIO-74001: 100 x 20µl Reactions: 1 x 1ml BIO-74005: 500 x 20µl Reactions: 5 x 1ml

Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2, ROXSYBR® Green I; Plus separate vials of: Reverse transcriptaseRNase inhibitorDEPC-treated water

Kit Size: The kit size is based on a 20µl final reaction volume.

Storage Conditions: 12 months at -20°C. Avoid exposure of the SYBR® Green I to light.