Features and Benefits
- Rapid - Optimized for fast reverse transcription real-time PCR
- Accurate quantification - For RNA from low copy targets
- Sensitive – unique buffer chemistry for highest specificity
- Flexible - Compatible with all fast cycling instruments
Instrument Compatibility: Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®.
The SensiFAST™ Probe No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube . The kit is designed for use with probe-detection technology , including TaqMan®, Scorpions® and molecular beacon probes . A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR. The SensiFAST Probe No-ROX One-Step Kit consists of a 2x SensiFAST Probe No-ROX One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.
Volume BIO-76001: 100 x 20µl Reactions: 1 x 1ml BIO-76005: 500 x 20µl Reactions: 5 x 1ml
Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2; Plus separate vials of: Reverse Transcriptase, RNase inhibitor, DEPC-treated water
Kit Size: The kit size is based on a 20µl final reaction volume.
Storage Conditions: 12 months at -20°C.
This product has met the following criteria: