Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor , with highly efficient and sensitive transcription, from as little as 10pg, up to 2mg of RNA. Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.
Features
- Unrivalled stability
- Working temperature range 37°- 45°C
- Highly sensitive for enhanced cDNA yield
- Produces high quality cDNA ideal for real-time PCR
- Reverse transcribes RNA templates up to 9Kb
Applications
- Unrivalled stability
- First strand cDNA synthesis for real-time PCR
- cDNA library construction
- mRNA 5’ end mapping by primer extension
- Dideoxynucleotide sequencing
- End-labelling of DNA
Components:Tetro Reverse Transcriptase, 5x Reaction Buffer, Concentration 200u/µl
Storage Conditions: Tetro Reverse Transcriptase can be stored for 12 months at -20°C.
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