Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single– or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM). Meridian Bioscience UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Applications
- UDG treatment of uracil-containing DNA to prevent amplification by DNA polymerases
- Used to investigate features of protein – DNA interactions
- Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli
- Components: Uracil DNA Glycosylase, 10x Reaction Buffer
- Unit Definition: One unit is the amount of enzyme that catalyzes the release of 60µmol of uracil per minute from uracil-containing double-stranded DNA
- Concentration: 1u/µl
- Storage Conditions Uracil DNA Glycosylase can be stored for 6 months at –20°C
This product has met the following criteria to qualify for the following awards:
 | Sustainable Product Manufacturing | - Measures were in place during the manufacture of this product to reduce water usage
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 | Sustainable Distribution & Packaging | - Country of origin has an Environmental Performance Index (EPI) greater than 60
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