Application

Estimation of the yield of DIG-labeled nucleic acids or as a control-hybridization probe in Southern blots.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Determination of the yield of digoxigenin (DIG)-labeled DNA is most important for optimal and reproducible hybridization results. Too high of a probe concentration in the hybridization step causes background, while too low of a concentration leads to weak signals.DIG-labeled control has a standardized concentration and can be used as a standard for comparison to experimentally generated DIG-labeled probes.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution, digoxigenin-labeled pBR328 DNA, 5 µg/ml

Quality

0.1pg of the DIG-labeled Control DNA can be visualized in a spot test with direct color detection.