Application

High concentration, DNase-free RNase has been used for:Genomic DNA isolationIsolation of plasmid DNADegradation of RNA for flow cytometry

General description

A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.

Preparation Note

Working concentration: 50 µg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl₂, 50% glycerol (v/v), pH 7.0

Quality

Absence of DNase activity tested according to the current Quality Control procedures.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A₀ to A₁ within 1 minute under assay conditions: A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.