Application

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

General description

Formula: C₂₀H₃₇N₃O₁₃ LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.) Molecular weight: Mr = 527.5 Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Other Notes

Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 µm pore size membrane.

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 µm pore size membrane

Preparation Note

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.

Quality

Purity: >80% (HPLC)

Storage and Stability

Store at 2 to 8 °C. (solution)

Unit Definition

Unit Assay: Unit/mgWe do not have this information.