Application

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mixfor the determination of kinetic characteristics using an enzyme-linked kinetic assay.

NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

Biochem/physiol Actions

The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

Electron acceptor

General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD⁺ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Other Notes

For life science research only. Not for use in diagnostic procedures.

This is the common form of NAD.

Packaging

Packaged by solid weight.

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Quality

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A₂₅₀nm - A₂₆₀nm : 0.83 ± 0.02, A₂₈₀nm - A₂₆₀nm : 0.22 ± 0.02Absence of contaminants: LDH inhibitors are not detectableOrganic solvents: =0.05% (GC)

Specifications

Absorbance coefficients: 17.6 x 10³l x mol^-1 x cm^-1 at 260nm, 5.9 x 10³l x mol^-1 x cm^-1 at 333nm (CN complex)