Application

Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting.

Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H₂O₂ produced from disaggregated slug cells.

Biochem/physiol Actions

Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives.

General description

Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables.

Donor:hydrogen-peroxide oxidoreductase

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents.Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml).

Quality

Contaminants: ﹤0.001% ATPase and acid phosphatase each, ﹤0.7% catalasePurity number: approximately 3.0 (A₄₀₃/A₂₇₅)

Reconstitution

Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0.