Application

5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cellsto label cleaved DNA ends in apoptosis assay in avian tissue sectionsfor incorporation into mice prostates for immunohistochemistry studies

General description

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.

Packaging

1, 5 mg in glass bottle

25 mg in poly bottle