Analysis Note

Absorbance coefficients: 15.0 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.3 x 10³ l x mol^-1 x cm^-1 at 340 nm

Application

NADPH-RO has been used for the determination of thioredoxin reductase activity.

Biochem/physiol Actions

NADPH is an electron donor and a cofactor for many redox enzymes including nitric oxide synthetase.

General description

Absorbance coefficients: 15.0 x 103 l x mol-1 x cm-1 at 260 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm Absorbance maxima: 260 nm and 340 nm Molecular weight: NADPH-Na4: Mr = 833.4, NADPH: Mr = 745.4 pH value in water: 8.0 ± 1.0 (10 mg/ml)

Amorphous powder bottled under nitrogen.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaged based on solid weight.

Preparation Note

Storage conditions (working solution): 2 to 8 °C10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADPH after 4 weeks at 2 to 8 °C.

Quality

Purity: =97% ß-NADPH-Na4 based on dry weight (enzymatically and absorbance at 340 nm), =80% (absorbance at 260 nm) Organic solvents: =3% (GC)