Analysis Note

Absorption: Content is measured in aqueous solution by absorption at 254 nm.Conditions: approx. 10 mg/ml double-dist. water dilution: 9.8 ml double-dist. water + 0.2 ml sample.

Application

GTP-γ-S has been used: in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα) to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants

Biochem/physiol Actions

Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),

General description

Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage and Stability

Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.