Analysis Note

Colonies appear clear to creamy white, some may produce pigment

Target Microorganisms: Total Count

QC organismsE. coli (ATCC 25922)S. epidermidis (ATCC 14990)

Application

m-HPC broth is used in the recovery of stressed heterotrophic bacteria found in various types of water. Also suitable for water samples with low counts. Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Culture Medium

m-HPC

General description

Heterotrophs are organisms including bacteria, yeasts and moulds that require an external source of organic carbon for growth. The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live heterotrophic bacteria in water.{14} This test can provide useful information about water quality and supporting data on the significance of coliform test results. Heterotrophic Plate Count Broth can be employed for the determination of bacteria by the membrane filter method. Peptic digest of animal tissue is the source of nutrients for organisms, which are not highly fastidious. Gelatin is utilized by microorganisms through a proteolytic mechanism. The addition of glycerol to the basal medium provides a source of carbon and energy

Legal Information

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50×2 mL in ampoules

2 mL plastic ampoules

Preparation Note

Prior to use, warm media at 30-50 °C until liquefied. Do not microwave.

Specifications

Incubation Temperature: 30–35 °C

Standard Methods for the Examination of Water and Wastewater

Incubation Time: 48–72 hrs