Analysis Note

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Application

The HY-LiTE^® Plus Pen is intended for detection of ATP in samples where higher than usual levels of inhibition are suspected, such as paints and coatings, Metal Working fluids, Hydraulic fluids, activated sludge (wastewater treatment) and similar.

General description

The HY-LiTE^® Plus pen is a ready prepared test designed for use in a HY-LiTE^® 2/2A luminometers and consists of the following parts: A protection cap with integrated dilution solution. A white sampling stick (covered by the protection cap) for accurate sampling of the liquid to be tested. The stick is coated with an extractant, which releases ATP from cellular material. The stick also transfers the sample into the cuvette and in a later step opens the reagent chamber. A cuvette filled with test buffer for dilution, buffering and neutralization of the sample. A reagent chamber sealed with aluminum foil, containing freeze-dried and stabilized Luciferin/Luciferase mixture.The test is based on firefly bioluminescence, using reagents especially formulated to compensate for high levels of inhibitory compounds, which are often encountered in complex organic aqueous matrices like paint emulsion, anti-corrosion coatings, metal working fluids etc. The test also includes a tube with dilution medium and a disposable pipette, enabling the user to perform a pre-dilution of the sample to further reduce inhibition. The dilution step is not mandatory, but it is recommended to perform at least a 1:3 sample dilution unless it has already been validated.

Legal Information

HY-LITE is a registered trademark of Merck KGaA, Darmstadt, Germany

Suitability

For detection of ATP in Industrial samples where higher than usual levels of inhibition are suspected