A medium recommended by the Harmonised USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonised USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A colour change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonised USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.
- Selective medium designed to recover Escherichia coli
- Described in the European Pharmacopoeia (EP) 2.6.13 and United States Pharmacopeia (USP) 62>
- Storage temperature: 2 to 25°C
- Appearance: clear, purple liquid
- Recommended incubation: 37/44°C
- pH: 7.1 to 7.5
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