EO Labs Tryptone Soya Agar- 500g

Tryptone Soya Agar is used for a wide range of applications, including culture storage, enumeration of cells, isolation of pure cultures, or simply general culture. It has been found to be useful in cosmetic testing, water, and wastewater applications. Tryptone Soya Agar may be used to determine X and V factor requirements of Haemophilus species; sterility testing; and environmental monitoring within pharmaceutical cleanrooms and sterile facilities. This medium meets the requirements of the Harmonised USP/EP/JP and is based on the original formulation described by Leavitt et al. in 1955. Tryptone Soya Agar is recommended as a reference medium when testing selective media, to measure the degree of inhibition. In environmental monitoring applications, it is common for plates to be incubated at 30 to 35°C for bacterial colonies and 20 to 25°C for mould and fungi. Tryptone Soya Agar will support the growth of both aerobic and anaerobic organisms depending on incubation conditions.

Unsupplemented Tryptone Soya Agar is recommended by the World Health Organisation, UK Standards for Microbiology Investigations, International Organisation for Standardisation and is tested in accordance with ISO 11133:2014. This medium is also included in the Bacteriological Analytical Manual for cosmetics testing. UK Standards for Microbiology Investigations also call for Tryptone Soya Agar supplemented with 5% sheep blood (E&O DSC) for aid in the identification of Bordetella species from clinical specimens.

The addition of defibrinated animal blood to the base medium, promotes the growth of most fastidious organisms and presumptive identification can be made based on haemolytic reactions. It should be noted that the haemolytic patterns of isolates may vary with the source of animal blood. Addition of selective agents allows isolation and presumptive identification of specific species or groups of organisms.

• Storage temperature: 10 to 30°C
• Dehydrated medium appearance: fine powder
• Prepared medium appearance: firm gel
• Dehydrated medium colour: pale straw
• Prepared medium colour: various depending on additives
• Recommended incubation: as required by organisms according to the methodology being followed
• pH: 7.3 ± 0.2