This is one of two media generally used for the selective isolation of Bordetella pertussis. The medium is made selective by the inclusion of cephalexin, to suppress the unwanted naso-pharyngeal flora often present in specimens submitted for the isolation of Bordetella pertussis, and further enriched with 10% horse blood.
NB: Although coliforms are inhibited by this medium, Pseudomonas aeruginosa and some fungi will grow.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: black
- Recommended incubation: aerobically at 37°C ± 1°C for up to 5 days
- pH: 7.4 ± 0.2
- Plate size: 90mm
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