The United States Pharmacopeia has updated the USP/NF Microbiological Test 60 Microbiological Examination of Non-Sterile Products - Tests for Burkholderia Cepacia Complex (Bcc) to include the use of this medium. This test is designed to determine whether a substance or preparation complies with an established specification for microbiological quality and/or to evaluate whether product contains members of the Bcc. Burkholderia Cepacia Selective Agar, Complete is the complete medium formulation specified by the USP/NF. BCSA, Complete contains peptones and sugars that supply nutrients for the growth of the Bcc. Phenol Red is the sugar utilisation indicator. Crystal Violet is added to inhibit the growth of Gram-positive bacteria. Antimicrobics are added to suppress or inhibit the recovery bacteria other than the Bcc. Agar is the solidifying agent.
- Analyte: burkholderia cepacia
- Platform: dehydrated culture media
- Formula: enzymatic digest of casein 10.0g/L, lactose 10.0g/L, sucrose 10.0g/L, sodium chloride 5.0g/L, yeast extract 1.5g/L, phenol red 0.08g/L, crystal violet 0.002g/L, vancomycin 0.0025g/L, gentamicin 0.01g/L, polymyxin B 600,000 IU/L, agar 14.0g/L
- Grams per litre: 50.69
- Litres per 500g: 9.90
- Plates per 500g: 657.60
This product has met the following criteria to qualify for the following awards: