Application

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.

Features and Benefits

Faster destaining than with alternative in-gel digest kits Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest Compatible with Coomassie, SYPRO^® Orange, SYPRO Ruby, and ProteoSilver^™ Stained gels Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

General description

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

SYPRO is a registered trademark of Life Technologies