Analysis Note

To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

Application

iTRAQ^® Reagent - 8PLEX Multiplex Kit has been used:to derivatize and label the tryptic digests of the iodoacetamide probe (IPM)-labeled HeLa proteomein isobaric tags for relative and absolute quantitation (iTRAQ) labeling of peptidesas isobaric labels for the comparative quantification of proteins between the digested samples in proteomic analysis

Features and Benefits

Simultaneously identify and quantify proteins from multiple samples Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs) Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein Increases throughput and confidence in results for protein biomarker discovery studies Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms

General description

Contains iTRAQ^® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately.Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry^® product number 4390812.

The isobaric tags for relative and absolute quantitation (iTRAQ^®) reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modification (PTM) information. The iTRAQ Reagents - 8plex kits are available in more economical bulk packs.

Legal Information

iTraq is a registered trademark of AB Sciex Pte. Ltd.

iChemistry is a registered trademark of AB Sciex Pte. Ltd.