Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Automated pilot scale purification of synthetic oligonucleotides; AKTAexplorer Summary This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.

Features and Benefits

SOURCE^™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up. Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes RESOURCE^™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE^™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min. Improved capacity compared to MonoBeads but with a slightly lower resolution.

General description

RESOURCE^™ Q columns are prepacked with SOURCE^™ 15Q, a strong anion exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules.The small (15 µm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE^™ 30Q and 30S media with a 30 µm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

Legal Information

RESOURCE is a trademark of Cytiva

SOURCE is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)