Mirsky's Fixative is a superior fixing agent for use in immunohistological and immunocytological protocols. Mirsky's Fixative contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odour. Mirsky's Fixative is neutral, buffered, and isotonic (308 milliosmoles). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent crosslinking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.

Mirsky's Fixative is normally distributed as a concentrated two bottle system (NAT1304/NAT1306), although high throughput laboratories often use the single bottle ready-to-use format (NAT1302/NAT1300).

The two-bottle system is comprised of Mirsky's Fixative 10X Concentrate and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.

The two-bottle system has a shelf life of 12 months while the ready-to-use format has a shelf life of 30 days.

• Saponin/Glyoxylate fixative
• Preserves immunohistological activity
• Contains no formaldehyde
• Fast-acting