Application

SYBR^® Green I nucleic acid gel stain has been used for: the quantification of dsDNA to stain DNA in polymerase chain reaction (PCR) for comet assay technique to assess spermatozoon membrane integrityfor visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP) as a fluorescent dye in flow cytometryreal-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

Features and Benefits

An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps It is less mutagenic than ethidium bromide in Ames tests It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides Useful for many applications with a limited amount of DNA The binding of SYBR^® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I Removal of this stain in-gel digestion and ligation techniques is not needed SYBR Green I is a greener alternative product to ethidium bromide for staining

General description

SYBR^® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Legal Information

SYBR is a registered trademark of Life Technologies

U.S. Patent No. 5,436,134. Licensed from Molecular Probes, Inc.

Storage and Stability

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.