Ethidium homodimer I solution, >=95%, 2 mM in DMSO

Code: E1903-.5ML D2-231

Application

Used to detect DNA in solution.

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Stains ssDNA, dsDNA, RNA, oligonucl...


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Application

Used to detect DNA in solution.

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.

Biochem/physiol Actions

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

ε (extinction coefficient)≥9000 at 490-496 nm in water
application(s)diagnostic assay manufacturinghematologyhistology
assay≥95%
colorred
concentration2 mM in DMSO
formliquid
InChI keyZKAJUKABUUJRLB-UHFFFAOYSA-N
InChI1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
Quality Level100
SMILES stringCl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
storage temp.−20°C
Cas Number61926-22-5
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