7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin, >=95% (HPLC), solid

Application

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin has been used:•as a nontoxic substitute for conjugation with antibody(93)•to aid fluorescent detection of Coenzyme A (CoA-SH) in human N-myristoyltransferases assay (94)•in thermostability shift assay of recombinant protein(95)

Biochem/physiol Actions

Used to monitor release of thiols, quantitate thiol in microplate reactions, and to distinguish proliferating cancer cells by nucleolar protein staining.

General description

7-Diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (CPM), is a highly fluorescent, sulfhydryl (-SH) group containing coumarin derivative. It has excitation an emission wavelengths of 355 and 460 nm, respectively.