Application
Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.
General description
Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing.
Reconstitution
Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing.
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