Application

Trypan blue is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

General description

Trypan Blue is a tetrasulfonated anionic, hydrophilic dye.

Physical form

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic