Nuclear Fast Red, for microscopy (Bot., Hist.)

Application

Used as a biological stain

Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include: staining sperm and spermatids in semen identifying areas for PCR analysis in paraffin-embedded tissues nuclear counterstaining following immunostaining and silver staining

Biochem/physiol Actions

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

General description

Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt.