Trypan Blue, Dye content 60 %

Code: 302643-25G D2-231

Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Biochem/physiol Actions

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€81.50 25G
€100.25 inc. VAT
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Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Biochem/physiol Actions

Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated anionic dye. It is also used to screen the cytotoxic properties of plant extracts. In addition, trypan blue is used for staining epiretinal membranes during vitrectomy surgery.

Packaging

25, 100 g in glass bottle

ε (extinction coefficient)60000 at 603-607 nm in methanol, ≥60000 at 603-607 nm in methanol at 0.01 g/L
λmax607 nm
application(s)diagnostic assay manufacturinghematologyhistology
compositionDye content, 60%
formpowder
Gene Informationhuman ... PTPN1(5770), PTPRF(5792)
InChI keyGLNADSQYFUSGOU-UHFFFAOYSA-J
InChI1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
mp>300 °C (lit.)
Quality Level200
SMILES string[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
solubilityH2O: 1 mg/mL
storage temp.room temp
Cas Number72-57-1
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