Sulforhodamine B, Dye content 75 %

Code: 230162-5G D2-231

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

General description<...


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Your Price
€177.00 5G
€217.71 inc. VAT

Application

Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.

General description

Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.

Packaging

1, 5 g in glass bottle

ε (extinction coefficient)≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
λmax554 nm
application(s)diagnostic assay manufacturinghematologyhistology
compositionDye content, 75%
formpowder
InChI keySXQCTESRRZBPHJ-UHFFFAOYSA-M
InChI1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
Quality Level100
SMILES string[Na+].CCN(CC)c1ccc2c(OC3=CC(C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](CC)CC)c1
solubilitymethanol: 1 mg/mL
storage temp.room temp
Cas Number3520-42-1
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