Application

L-Leucine Dehydrogenase from Bacillus cereus has been used to determine the branched-chain amino acids (BCAA) spectrophotometrically in serum samples.

Biochem/physiol Actions

Leucine Dehydrogenase is a nicotinamide adenine dinucleotide hydrogen (NADH)-dependent oxidoreductase. It is involved in catalyzing the reductive amination of aliphatic 2-oxo-acids to their respective L-amino acids.

General description

L-Leucine Dehydrogenase is a member of the amino acid dehydrogenase family.

Other Notes

contains lysine

Unit Definition

One unit will convert 1.0 µmole of L‑leucine to α-ketoisocaproate per min at pH 10.5 at 37 °C.