alpha-Galactosidase from green coffee beans, ammonium sulfate suspension, >=9 units/mg protein

Application

α-Galactosidase from green coffee beans has been used to assess the specificity of anti- α-Galactosidase antibodies. It has also been used in inhibition assay to assess N-guanidino derivatives of 1,5-dideoxy-1,5-imino-d-xylitol activity.

Biochem/physiol Actions

α-Galactosidase from green coffee beans cleaves the terminal α-galactose from the oligosaccharides. α-Galactosidase deficiency is associated with Fabry disease.

General description

α-Galactosidase from green coffee beans is encoded by the GLA gene.

Packaging

25 units in glass bottle

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 6.0, containing BSA

Unit Definition

One unit will hydrolyze 1.0 µmole of p-nitrophenyl α-D-galactoside to p-nitrophenol and D-galactose per min at pH 6.5 at 25 °C.