beta-Galactosidase from Escherichia coli, Grade VI, lyophilized powder, 250-600 units/mg protein

Code: G6008-1KU D2-231

Application

β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporte...


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€160.50 EACH
€197.42 inc. VAT

Application

β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

General description

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Packaging

10000 units in poly bottle

1000, 5000 units in glass bottle

Physical form

Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 µmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

biological sourceEscherichia coli
compositionProtein, ≥50% biuret
does not containBSA as extender
formlyophilized powder
mol wt465 kDa
Quality Level200
shipped inwet ice
specific activity≥250 units/mg protein
storage temp.−20°C
typeGrade VI
Code
Description
Unit Size
List Price
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Cas Number9031-11-2
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