Application

Dextranase from Sigma has been used for the hydrolysis of carbohydate polymers, during the study of polysaccharide synthesis by Phanerochaete chrysosporium. It has also been used in the synthesis of new enzymatically degradable thermo-responsive nanogels.

Dextranase from Penicillium sp. has been used in a study to assess a new method of synthesizing biopolymeric affinity ligands. Dextranase from Penicillium sp. Has also been used in a study to investigate the cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum.

Biochem/physiol Actions

An endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

5000 units in poly bottle

Quality

Crude

Unit Definition

One unit will liberate 1.0 µmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.