Application

Cellulase from Aspergillus niger has been used:in the preparation of the standardized solution to determine absolute cellulase levelsin phosphate-citric acid buffer for activity assayin non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Unit Definition

1 U corresponds to the amount of enzymes which liberates 1 µmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C