Application

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Caution

May darken in storage.

General description

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H₂SO₄ and read at 492 nm.

Packaging

50, 100 g in glass bottle