RV1MAG-26K-02

Application

Analytes: Caveolin-1, CINC-1/GRO/KC, CTGF (Connective Tissue Growth Factor), IL-6, MCP-1, PAI-1 (total), TIMP-1, TNF-α, VEGF Recommended Sample type: serum and plasma Recommended Sample dilution: 1:4 in kit Serum Matrix Assay Run Time: Overnight Research Category: Toxicity

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Vascular injury is important to address during the preclinical safety study of drugs. A lack of well-defined mechanisms of drug-induced vascular injury (DIVI) in animals, and the absence of specific, qualified biomarkers have become significant barriers in the development of new therapeutic treatments. In the past, hemodynamics, such as heart rate and mean arterial blood pressure, were used to measure DIVI risks but these only targeted single biomarkers. Today, some of the known vascular injury mechanisms are related to vascular wall shear and/or circumferential “hoop” stress, drug/chemical toxicity, and immunological mediation. With these known biomechanics, primary targets (smooth muscle, endothelial and immune-mediated cells) yield a number of promising candidate biomarkers for DIVI study. We provide valuable research assays to investigate multiple biomarkers of vascular injury in rat serum and plasma samples using the Luminex^® xMAP^® instrument platform. The MILLIPLEX^® Rat Vascular Injury Bead Panel contains all the components necessary to measure the following 9 biomarkers in any combination using Luminex^® xMAP^® technology: Caveolin-1, CINC-1/GRO/KC, CTGF (Connective Tissue Growth Factor), IL-6, MCP-1, PAI-1 (total), TIMP-1, TNFα, VEGF. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate. Panel Type: Toxicity

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: See kit protocol for individual analytes sensitivities.

Please contact Technical Service for linearity of dilution.

Packaging

Everything you need in a single kit.

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

assay range	standard curve range: 0.3-200 ng/mL(CTGF), accuracy: 97-108%, inter-assay cv: ﹤20%(Caveolin-1), inter-assay cv: ﹤10%(All other analytes), standard curve range: 0.2-150 ng/mL(TIMP-1), standard curve range: 0.1-100 ng/mL(CINC-1/GRO/KC, IL-6, MCP-1, PAI-1 (total)), inter-assay cv: ﹤15%(CINC-1/GRO/KC, PAI-1 (total), TIMP-1, TNFα), standard curve range: 1.4-1,000 ng/mL(Caveolin-1), standard curve range: 0.1-20 ng/mL(TNFα), intra-assay cv: ﹤10%, standard curve range: 0.1-15 ng/mL(VEGF)
detection method	fluorometric (Luminex xMAP)
manufacturer/tradename	Milliplex^®
Quality Level	200
shipped in	wet ice
species reactivity	rat
technique(s)	multiplexing: suitable