Application

Analytes: Adiponectin, IL-1β, IL-6, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα Recommended Sample Type: Rat cell/tissue culture supernatant or lysates. Recommended Sample Dilution: 25 µL per well of cell/Tissue culture sample. Samples may require a dilution with an appropriate control medium. Assay Run Time: Overnight (16-18 hours) at 2-8°C Research Category: Metabolism Research Subcategory: Endocrine; Obesity; Metabolic Disease

Components

Rat Adipokine Standard, 1 vial, lyophilizedRat Adipokine Quality Control 1, 1 vial, lyophilizedRat Adipokine Quality Control 2, 1 vial, lyophilizedAssay Buffer, 1 bottle, 30 mLWash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL eaRat Adipocyte Detection Antibodies, 1 bottle, 5.5 mLStreptavidin-Phycoerythrin, 1 bottle, 5.5 mLMixing Bottle, 1 bottleSample Plate, 96 well format, 1 plate2 Foil Plate Sealers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body’;s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack. The MILLIPLEX^® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Metabolism

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sensitivity: Please see kit protocol for individual assay sensitivities.

Please contact Technical Service for linearity of dilution.

Packaging

Everything you need in a single kit.

Specificity

Cross ReactivtyThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.